Journal: Cells
Article Title: Effects of Scaffolds on Urine- and Urothelial Carcinoma Tissue-Derived Organoids from Bladder Cancer Patients.
doi: 10.3390/cells12162108
Figure Lengend Snippet: Figure 2. Analysis of marker gene expression in RT112 spheroids as a function of the scaffold composition. The expression of p53 (top), TP63 (middle), and Ki67 (bottom) in RT112 cells cultured in BME−(left) or 0.2% GD (right) was investigated by immunofluorescence and labeled in red. Cell nuclei are indicated in blue (DAPI). The exposure time is indicated in the individual micrographs in milliseconds, objective 40×. Control stainings omitting the primary antibody are provided in the supplement. Abbreviations: BME, basal membrane extract; GD, GrowDex®.
Article Snippet: The BC cell line RT112 (AC488; available from DSMZ Leibnitz Institute Braunschweig, Germany; a generous gift from Prof. Peter Black, Vancouver Prostate Center) was expanded in DMEM (Thermo Fisher, Waltham, MA, USA) media enriched by 10% FBS and antibiotics as described [27].
Techniques: Marker, Gene Expression, Expressing, Cell Culture, Labeling, Control, Membrane